BG-11

For 1 liter of BG-11:
1.5 g of NaNO₃.
0.04 g of K₂HPO₄.
0.075g of MgSO₄.7H₂O.
0.036g of CaCl₂.2H₂O.
0.006g of citric acid.
0.006g of ferric ammonium citrate.
0.001g of disodium EDTA.
0.02g of Na₂CO₃.
Complete to 1 liter with water.
Autoclave.
Add 1 ml of sterile Metal mix A5.

Metal mix A5:
2.86g de H₃BO₃.
1.81g de MnCl₂.4H₂O.
0.222g de ZnSO₄.7H₂O.
0.39g Na₂MoO₄.2H₂O.
0.079g de CuSO₄.5H₂O.
0.049g de Co(NO₃)₂.6H₂O.
Filter sterilize.

For BG-11 agar:
Prepare 500ml of 2x BG-11 (use the quantities listed above for 1L), autoclave, and cool to 50°C.
Prepare 500ml of 2x agar, dissolve 15g of agar in 500ml of water d'eau, autoclave, and cool to 50°C.
Preparer a 1M stock solution of Na₂S₂O₃.5H₂O, filter sterilize.
Mix
- 500ml of sterile 2x BG-11.
- 500ml of steriles 2x agar.
- 1ml of sterile 1M Na₂S₂O₃.5H₂O.
- 1ml of sterile Metal Mix A5.
Pour plates quickly.